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Do transparent exopolymeric particles (TEP) affect the toxicity of nanoplastics on Chaetoceros neogracile? ArchiMer
González-fernández, Carmen; Toullec, Jordan; Lambert, Christophe; Le Goïc, Nelly; Seoane, Marta; Moriceau, Brivaela; Huvet, Arnaud; Berchel, Mathieu; Vincent, Dorothée; Courcot, Lucie; Soudant, Philippe; Paul-pont, Ika.
The potential presence of nanoplastics (NP) in aquatic environments represents a growing concern regarding their possible effects on aquatic organisms. The objective of this study was to assess the impact of polystyrene (PS) amino-modified particles (50  nm PSNH2) on the cellular and metabolic responses of the diatom Chaetoceros neogracile cultures at two essential phases of the growth cycle, i.e. exponential (division) and stationary (storage) phases. Both cultures were exposed for 4 days to low (0.05 μg mL−1) and high (5 μg mL−1) concentrations of PS-NH2. Exposure to NP impaired more drastically the major cellular and physiological parameters during exponential phase than during the stationary phase. Only an increase in ROS production was observed at...
Tipo: Text Palavras-chave: Nanoplastics; Diatoms; Physiology; Transparent exopolymer particles.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00491/60235/63627.pdf
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External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease ArchiMer
Rahmani, Alexandra; Mathien, Clémentine; Bidault, Adeline; Le Goïc, Nelly; Paillard, Christine; Pichereau, Vianney.
Aims Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis. Methods and Results In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids...
Tipo: Text Palavras-chave: BRD; Modulation effect; Neutralizing activity; PH; PH modifications; Ruditapes philippinarum; Vibrio tapetis.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74142/73669.pdf
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Impact of nine macroalgal diets on growth and initial reproductive investment in juvenile abalone Haliotis tuberculata ArchiMer
Roussel, Sabine; Caralp, Claire; Leblanc, Catherine; Le Grand, Fabienne; Stiger-pouvreau, Valerie; Coulombet, Céline; Le Goïc, Nelly; Huchette, Sylvain.
The commercial culture of Haliotis tuberculata has recently started in Europe. As abalone is herbivorous, the use of local collected algae as feed may appear advantageous. The nutritional value of eight monospecific seaweed diets was studied using Palmaria palmata (Rhodophyta), filamentous algae, mainly Gracilaria sp. (Rhodophyta), Enteromorpha sp. and Ulva lactuca (Chlorophyta), together with Saccharina latissima, Saccorhiza polyschides, Laminaria digitata and Laminaria hyperborea (Ochrophyta, Phaeophyceae) and a mixed macroalgal diet. An integrative approach consisted in monitoring the seasonal composition changes of these algae in terms of protein, lipid, soluble carbohydrate, fatty acid and amino-acid contents, and to relate it to seasonal growth and...
Tipo: Text Palavras-chave: Abalone; Algae; Reproduction; Growth; Protein.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00509/62066/66246.pdf
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Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene.
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the...
Tipo: Text Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni..
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf
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